OBJETIVES:

• Define the main types of genetics tools used in Mycobacteria
• Apply experimental designs using genetic tools
• Evaluate aspects which contribute for the success of genetic manipulation of Mycobacteria

Topics:

• Terms Used in genetics
  • Gene nomenclature
• Plasmids: gene cloning and in vitro mutagenesis
• Gene Transfer
• Transformation: physiological and artificial
• Transduction and bacteriophages
• Homologous recombination
• Why use surrogates instead of Mycobacterium tuberculosis per se
• Analysis of mutant libraries
  • transposon mutagenesis
• Types of mutants
  • Auxotrophic mutants
  • Conditional mutants
  • Resistant mutants

REFERENCES

Gene nomenclature - Wikipedia

16. Experimental genetics of Mycobacterium tuberculosis (1).pdf

Mycobacteria recombineering.pdf

Introduction-compactado.pdf

Bacterial DNA Manipulations_compressed.pdf